missing translation for 'onlineSavingsMsg'
Learn More

CD3e, Functional Grade, clone: eBio500A2 (500A2), eBioscience™

Syrian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-0033-86

Additional Details : Weight : 0.01000kg

 View more versions of this product

Product Code. 15258347

  • 3555.00 SEK / 1mg


EU Stock 2
Add to basket

Description

Description

Description: The eBio500A2 monoclonal antibody reacts with the 25 kD epsilon subunit of the mouse CD3 complex. CD3 subunits gamma, delta and ε are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, as well as NKT cells. Binding of eBio500A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation. The 500A2 antibody is able to partially cross-block the 17A2 and 145-2C11 antibodies, indicating that all three of these anti-CD3 antibodies recognize distinct but overlapping epitopes.Applications Tested: This eBio500A2 (500A2) antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay. Purity: >;90% as determined by SDS-PAGE; Aggregation is <10% as determined by HPLC.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD3e
Monoclonal
1mg/mL
PBS with no preservative; pH 7.2
P22646
FLJ18683, T3E, TCRE, CD3-epsilon, CD3e antigen, epsilon polypeptide TiT3 complex, T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain
Golden Syrian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow CytometryþFunctional Assay
eBio500A2 (500A2)
Functional Grade
Cd3e
Purified
Cd3e
IgG
1mg
Store at 2-8°C.
Primary
12501
Videos

For Research Use Only.