CD4/CD25, APC, FITC, eBioscience™
Brand: Affymetrix eBioscience 22-0425-73
Additional Details : Weight : 0.23750kg
DescriptionDescription: This product consists of two fluorochrome-conjugated monoclonal antibodies to identify CD4+CD25+ human peripheral blood cells. The RPA-T4 monoclonal antibody (mouse IgG1, kappa) reacts with human CD4, a 59kDa cell surface receptor that is expressed by a majority of thymocytes, a subpopulation of mature T cells (T helper cells) and at low levels on monocytes. The BC96 monoclonal antibody (mouse IgG1, kappa) reacts with human CD25, the 55kDa interleukin-2 receptor alpha chain (IL-2Ralpha). This cocktail of RPA-T4 and BC96 can be used for the flow cytometric detection of human regulatory T cells, which also express Foxp3.Applications Reported: This product has been reported for use in flow cytometric analysis.Applications Tested: This cocktail has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20μL per test (containing 1μg of Anti-Human CD4 (RPA-T4) FITC and 0.125μg of Anti-Human CD25 (BC96) APC). A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
|PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2|
|CD4 antigen (p55), CD4 antigen p55, CD4 receptor, IL-2 receptor subunit alpha, IL-2R alpha, IL-2R alpha chain, IL-2R subunit alpha, IL2 RA antibody, Il2ra antibody, Interleukin 2 receptor alpha chain antibody, interleukin 2 receptor, alpha, T-cell surface antigen T4/Leu-3, TAC antigen, TAC antigen antibody|
|Affinity chromatography, Affinity chromatography|
|RPA-T4 (CD4) and BC96 (CD25)|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|3559, 3559, 920, 920|
For Research Use Only.