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CD4/CD25, APC, FITC, eBioscience™

Antibody Cocktail

Brand:  Affymetrix eBioscience 22-0425-73

Additional Details : Weight : 0.23750kg

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Product Code. 15508316

  • 3240.00 SEK / 100 tests


EU Stock 10
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Disclaimers:

For Research Use Only.


Description

Description

Description: This product consists of two fluorochrome-conjugated monoclonal antibodies to identify CD4+CD25+ human peripheral blood cells. The RPA-T4 monoclonal antibody (mouse IgG1, kappa) reacts with human CD4, a 59kDa cell surface receptor that is expressed by a majority of thymocytes, a subpopulation of mature T cells (T helper cells) and at low levels on monocytes. The BC96 monoclonal antibody (mouse IgG1, kappa) reacts with human CD25, the 55kDa interleukin-2 receptor alpha chain (IL-2Ralpha). This cocktail of RPA-T4 and BC96 can be used for the flow cytometric detection of human regulatory T cells, which also express Foxp3.Applications Reported: This product has been reported for use in flow cytometric analysis.Applications Tested: This cocktail has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20μL per test (containing 1μg of Anti-Human CD4 (RPA-T4) FITC and 0.125μg of Anti-Human CD25 (BC96) APC). A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Specifications

Specifications

CD4/CD25
RPA-T4 (CD4) and BC96 (CD25)
APC, FITC
Conjugated
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Human
Flow Cytometry
20μL/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
CD4 (RPA-T4); CD25 (BC96)
RUO
Monoclonal
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