missing translation for 'onlineSavingsMsg'
Learn More

CD40 Mouse anti-Human, Alexa Fluor 700, Clone: 5C3, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 56-0409-42

Additional Details : Weight : 0.01000kg

 View more versions of this product

Product Code. 16606434

  • 3540.00 SEK / 100 tests
Estimated Shipment: 24-08-2022
to see stock.

Please to purchase this item. Need a web account? Register with us today!


Description

Description

Description: The 5C3 monoclonal antibody reacts with human CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development and maturation by inducing Ig isotype-switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation. 5C3 is reported to be used for activation of B cells in in vitro functional assays. Applications Reported: This 5C3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 5C3 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm). Most instruments will require a 685 LP mirror and 710/20 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome. Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser. The CD40 antigen is a single chain glycoprotein that is known to be a member of the tumor necrosis factor/nerve growth factor superfamily and shows a significant homology to the Hodgkin′s disease associated antigen, CD30. CD40 is present on all B cells except plasma cells and is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of CD40, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with CD40 and serves as a mediator of the signal transduction. The interaction of CD40 and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of CD40 encoding distinct isoforms have been reported. Diseases associated with CD40 dysfunction include Type 3 Hyper-Igm immunodeficiency and CD40 ligand deficiency.
Specifications

Specifications

CD40
Monoclonal
5 μL/Test
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
P25942
CD40
Affinity chromatography
RUO
958
4°C, store in dark, DO NOT FREEZE!
Liquid
Flow Cytometry
5C3
Alexa Fluor 700
CD40
AI326936; B cell surface antigen CD40; B cell-associated molecule; B-cell surface antigen CD40; BOS_13299; Bp50; CD antigen CD40; CD40; CD40 antigen; CD40 antigen (TNF receptor superfamily member 5); CD40 antigen, TNF receptor superfamily member 5; CD40 molecule; CD40 molecule, TNF receptor superfamily member 5; CD40L receptor; CDw40; GP39; HIGM1; I79_006806; IGM; IMD3; Immunoglobulin M; ImmunoglobulinM; LOC100349106; membrane protein CD40; MGC9013; p50; sCD40; soluble CD40; T-BAM; T-cell differentiation antigen; Tnfrsf5; TRAP; Tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5
Mouse
100 Tests
Primary
Human
Antibody
IgG1 κ
Videos
SDS
Documents
Certificates
Special Offers

Special Offers