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CD40 Rat anti-Mouse, Clone: 1C10, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-0401-81

Additional Details : Weight : 0.01000kg

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Product Code. 15216937

  • 626.00 SEK / 50µg

Estimated Shipment
21-07-2021

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Description

Description

Description: The 1C10 monoclonal antibody reacts with mouse CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development/maturation by inducing Ig isotype switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation. The monoclonal antibody 1C10 is reported to have agonistic activity in vitro and in vivo. Applications Reported: This 1C10 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. (Please use Functional Grade purified 1C10, cat. 16-0401, in functional assays.). Applications Tested: The 1C10 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

The 1C10 monoclonal antibody reacts with mouse CD40, a 45-50kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development/maturation by inducing Ig isotype switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.

The monoclonal antibody 1C10 is reported to have agonistic activity in vitro and in vivo.
Specifications

Specifications

CD40
1C10
Unconjugated
Purified
Rat
50μg
Store at 2-8°C.
Monoclonal
Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
TNFRSF5
IgG2a, kappa
RUO
Primary
Murine
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For Research Use Only.