CD43, PerCP-eFluor™ 710, clone: 4-29-5-10-21, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 46-0438-41
Additional Details : Weight : 0.23750kg
DescriptionDescription: The monoclonal antibody 4-29-5-10-21 reacts with human and non-human primate CD43, which is also known as leukosialin, galactoglycoprotein and sialophorin. CD43 is a sialomucin which, like many mucins, can have both adhesive and anti-adhesive functions. Expression of CD43 is found on most leukocytes and a small subset of B lymphocytes. Proteolytic processing upon activation decreases surface expression. CD43 is involved in the activation of T cells, B cells, NK cells, and monocytes. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages.Applications Reported: This 4-29-5-10-21 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 4-29-5-10-21 antibody has been pre-titrated and tested by flow cytometric anaylsis of normal rhesus peripheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|