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CD45.2 Mouse anti-Mouse, Clone: 104, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-0454-85

Additional Details : Weight : 0.01000kg

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Product Code. 15226977

  • 2090.00 SEK / 500µg

Estimated Shipment
21-07-2021

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Description

Description

Description: The 104 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.2-expressing mouse strains. The strains that express CD45.2 include the most commonly used mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. CD45.2 is expressed by all leukocytes in these strains. Applications Reported: The 104 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. (Biotinylated 104 is recommended for IHC, cat. 13-0454). Applications Tested: The 104 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45.2 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45.2 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45.2 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
Specifications

Specifications

CD45.2
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
P06800
B220, CD45, CD45R, GP180, L-CA, LCA, LY5, T200, CD45 antigen, T200 glycoprotein, T200 leukocyte common antigen, protein tyrosine phosphatase, receptor type, c polypeptide, receptor-type tyrosine-protein phosphatase C, PTPRC
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation
104
Unconjugated
Ptprc
Liquid
Ptprc
IgG2a, kappa
500 μg
4° C
Primary
19264
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SDS
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For Research Use Only.