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CD45 Monoclonal Antibody (30-F11), Functional Grade, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Brand: Invitrogen 16-0451-050
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Description
The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes. Applications Reported: The 30-F11 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. 30-F11 has also been reported in complement-dependent cytotoxicity. (Please use Functional Grade purified 30-F11 in functional assays). Applications Tested: This 30-F11 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. This can be used at less than or equal to 0.5 ÎĽg per test. A test is defined as the amount (ÎĽg) of antibody that will stain a cell sample in a final volume of 100 ÎĽL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 ÎĽm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/ ÎĽg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.
CD45 Monoclonal antibody specifically detects CD45 in Mouse samples. It is validated for Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation
Specifications
| CD45 | |
| Monoclonal | |
| 1 mg/mL | |
| PBS with no preservative; pH 7.2 | |
| P06800 | |
| Rat | |
| 50 mg | |
| Primary | |
| Mouse | |
| Antibody | |
| IgG2b Îş |
| Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation | |
| 30-F11 | |
| Functional Grade | |
| PTPRC | |
| B220; Cd45; CD45 antigen; CD45 antigen isoform 1 precursor; CD45 antigen isoform 2 precursor; CD45 antigen isoform 3 precursor; CD45 antigen isoform 4 precursor; CD45 antigen isoform 5 precursor; CD45 antigen isoform 6 precursor; CD45R; GP180; LCA; L-CA; leucocyte common antigen; leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; leukocyte common antigen, CD45; loc; LOW QUALITY PROTEIN: receptor-type tyrosine-protein phosphatase C; LY5; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; membrane tyrosine phosphatase; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; protein tyrosine phosphatase; alternatively spliced; PTPRC; receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220 | |
| Affinity chromatography | |
| RUO | |
| 19264 | |
| 4° C | |
| Liquid |
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Product Title
CD45 Monoclonal Antibody (30-F11), Functional Grade, eBioscience™, Invitrogen™ > 50 mg; Functional Grade
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