CD45, eVolve 655, clone: 30-F11, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 86-0451-42
Additional Details : Weight : 0.23750kg
DescriptionDescription: The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.Applications Reported: This 30-F11 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 30-F11 antibody has been pre-titrated and tested by flow cytometric analysis of mouse bone marrow cells. This can be used at 5 μL per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.Laser/Filter Recommendation: eVolve™ 655 can be optimally excited with laser lines from 325-405 nm, and emits near 655 nm. We recommend using the violet (405 nm) laser line for excitation and a 630LP with a 660/40 bandpass filter, or equivalent, for detection.Fixation Recommendation: Antibodies conjugated to eVolve™ are compatible with eBioscience's fixation and permeabilization buffers.Excitation: 350-635 nm; Emission: 657 nm; Laser: Ultraviolet Laser, Violet LaserStorage and handling: For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.
|PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2|
|Cluster designation 45, common leukocyte antigen, T220 and B220|
|Store at 2-8°C. Do not freeze. Light-sensitive material. This product should be used within 6 months from the date of receipt.|
For Research Use Only.