missing translation for 'onlineSavingsMsg'
Learn More

CD45 Monoclonal Antibody (30-F11), PE-Cyanine5, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

1512.00 SEK - 3505.00 SEK

Specifications

Antigen CD45
Clone 30-F11
Concentration 0.2 mg/mL
Applications Flow Cytometry
Classification Monoclonal
View More Specs

Produkte 3
Produktcode Marke Quantity Preis Menge & Verfügbarkeit  
Produktcode Marke Quantity Preis Menge & Verfügbarkeit  
15350480
Unterlagen ansehen Aktionsdetails
Affymetrix eBioscience
15-0451-81
50 μg
1512.00 SEK
50µg
Alternative Produkte anzeigen
Sparen Sie bis zu 
»
Estimated Shipment: 09-04-2024
to see stock.
Please to purchase this item. Need a web account? Register with us today!
15360480
Unterlagen ansehen Aktionsdetails
Affymetrix eBioscience
15-0451-82
100 μg
2225.00 SEK
100µg
Alternative Produkte anzeigen
Sparen Sie bis zu 
»
In Stock
to see stock.
Please to purchase this item. Need a web account? Register with us today!
15370480
Unterlagen ansehen Aktionsdetails
Affymetrix eBioscience
15-0451-83
200 μg
3505.00 SEK
200µg
Alternative Produkte anzeigen
Sparen Sie bis zu 
»
Estimated Shipment: 07-05-2024
to see stock.
Please to purchase this item. Need a web account? Register with us today!
Beschreibung

Beschreibung

Description: The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes. Applications Reported: The 30-F11 antibody has been reported for use in flow cytometric analysis. Applications Tested: The 30-F11 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.
Spezifikation

Spezifikation

CD45
0.2 mg/mL
Monoclonal
Liquid
RUO
PBS with 0.09% sodium azide; pH 7.2
B220; cd45; CD45 antigen; CD45 antigen isoform 1 precursor; CD45 antigen isoform 2 precursor; CD45 antigen isoform 3 precursor; CD45 antigen isoform 4 precursor; CD45 antigen isoform 5 precursor; CD45 antigen isoform 6 precursor; CD45R; GP180; Lca; L-CA; leucocyte common antigen; leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; leukocyte common antigen, CD45; loc; LOW QUALITY PROTEIN: receptor-type tyrosine-protein phosphatase C; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; membrane tyrosine phosphatase; protein tyrosine phosphatase lambda; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; protein tyrosine phosphatase; alternatively spliced; Ptprc; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T
PTPRC
Primary
4° C, store in dark, DO NOT FREEZE!
PTPRC
30-F11
Flow Cytometry
PE-Cyanine5
Rat
Mouse
P06800
19264
IgG2b κ
Affinity chromatography
Antibody
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

Dokumentation

Product Certifications

For Research Use Only.