CD45R (B220), Biotin, clone: RA3-6B2, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 13-0452-86
Additional Details : Weight : 0.25000kg
DescriptionDescription: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.Applications Reported: This RA3-6B2 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of formalin-fixed paraffin embedded and frozen tissue sections.Applications Tested: The RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|B220, CD45R, Cd45, Ly-5, Lyt-4, T200, Cluster designation 45R|
|Flow CytometryþImmunohistochemistry (Frozen)þImmunohistochemistry (Paraffin)|
|IgG2a, kappa, kappa|
|Store at 2-8°C. Do not freeze.|
For Research Use Only.