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CD45R (B220), clone: RA3-6B2, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-0452-82

Additional Details : Weight : 0.01000kg

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Product Code. 15246967

  • 658.00 SEK / 100µg


EU Stock 5
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Description

Description

Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.Applications Reported: The RA3-6B2 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen or formalin-fixed paraffin embedded tissue sections. RA3-6B2 has also been reported in functional studies of B cells. (Please use Functional Grade purified RA3-6B2, cat. 16-0452, in functional assays.).Applications Tested: The RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The RA3-6B2 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low and high pH antigen retrieval and can be used at less than or equal to 5 μg/mL. The RA3-6B2 antibody has been tested by immunohistochemistry of frozen mouse tissue and can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD45R (B220)
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P08575, P06800
B220, CD45R, Cd45, Ly-5, Lyt-4, T200, Cluster designation 45R
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Human, Murine
Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation
RA3-6B2
Unconjugated
PTPRC
Liquid
Ptprc
IgG2a, kappa, kappa
100μg
4° C
Primary
19264, 5788
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