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CD47, clone: miap301, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-0471-82

Additional Details : Weight : 0.01000kg

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Product Code. 15236987

  • 930.00 SEK / 100µg


Estimated Shipment date
from Supplier 22-11-2018
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Description

Description

Description: The monoclonal antibody miap301 reacts to CD47 also known as integrin-associated protein (IAP), and neurophilin. CD47 is a glycosylated five transmembrane protein with a small cytoplasmic domain. CD47 is involved in adhesion through interactions with SIRP (signal regulator protein) and is non-covalently associated with β3 integrins CD51/CD61, CD41/CD61. It is also known to be the receptor for thrombospondin. T cell expression of CD47 can mediate activation or apoptosis (in the presence of high levels of thrombospondin). Expression is found in the majority of hematopoietic cells including T and B cells, monocytes, platelets and erythrocytes (as part of the Rh complex). Expression is also found in non-hematopoietic cells.No crossreactivity is seen against human CD47.Applications Reported: This miap301 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB) under nonreducing conditions, and immunohistology staining of frozen tissue sections.Applications Tested: This miap30 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD47
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
Q61735
IAP, MER6, OA3, CD47 antigen, CD47 glycoprotein, Rh-related antigen, leukocyte surface antigen CD47
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot
miap301
Unconjugated
Cd47
Liquid
Cd47
IgG2a, kappa, kappa
100μg
4° C
Primary
16423
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