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CD47, clone: miap301, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-0471-85

Additional Details : Weight : 0.01000kg

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Product Code. 15246987

  • 2030.00 SEK / 500µg


EU Stock 1
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Description

Description

Description: The monoclonal antibody miap301 reacts to CD47 also known as integrin-associated protein (IAP), and neurophilin. CD47 is a glycosylated five transmembrane protein with a small cytoplasmic domain. CD47 is involved in adhesion through interactions with SIRP (signal regulator protein) and is non-covalently associated with β3 integrins CD51/CD61, CD41/CD61. It is also known to be the receptor for thrombospondin. T cell expression of CD47 can mediate activation or apoptosis (in the presence of high levels of thrombospondin). Expression is found in the majority of hematopoietic cells including T and B cells, monocytes, platelets and erythrocytes (as part of the Rh complex). Expression is also found in non-hematopoietic cells.No crossreactivity is seen against human CD47.Applications Reported: This miap301 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB) under nonreducing conditions, and immunohistology staining of frozen tissue sections.Applications Tested: This miap30 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD47
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
Q61735
IAP, MER6, OA3, CD47 antigen, CD47 glycoprotein, Rh-related antigen, leukocyte surface antigen CD47
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot
miap301
Unconjugated
Cd47
Liquid
Cd47
IgG2a, kappa, kappa
500μg
Store at 2-8°C.
Primary
16423
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