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CD49a (Integrin alpha 1), PerCP-eFluor™ 710, clone: TS2/7, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-9490-41

Additional Details : Weight : 0.23750kg

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Product Code. 15519426

  • 966.00 SEK / 25 tests


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Description

Description

Description: The monoclonal antibody TS2/7 reacts with human CD49a, also known as integrin alpha 1. CD49a is always associated with CD29 (integrin beta 1) to form a type I transmembrane protein called very late antigen-1 (VLA1). This heterodimer is expressed by endothelial cells, activated T cells, monocytes, a subset of NK cells, a subset of memory T cells, smooth muscle cells, and mesenchymal stem cells. CD49a binds to collagen IV strongly, but also binds to collagen I, laminin, and semaphorin7A. CD49a is involved in cell survival, proliferation, homing, and inflammatory responses.Applications Reported: This TS2/7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This TS2/7 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD49a (Integrin alpha 1)
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P56199
ITGA1, VLA1
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
TS2/7
PerCP-eFluor 710
ITGA1
Conjugated
ITGA1
IgG1, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
3672
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