CD49d (Integrin alpha 4), PE, clone: R1-2, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 12-0492-83
Additional Details : Weight : 0.25000kg
DescriptionDescription: The R1-2 monoclonal antibody reacts with mouse CD49d, the 150kDa integrin alpha4 subunit. The complex of CD49d non-covalently associated with integrin beta1 (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106). This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils. CD49d also associates with integrin b7 and binds to the Mucosal vascular Addressin (MAdCAM-1).Applications Reported: The R1-2 antibody has been reported for use in flow cytometric analysis.Applications Tested: The R1-2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|CD49d (Integrin alpha 4)|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|269C wild type, CD49 antigen-like family member D, VLA-4 subunit alpha, antigen CD49D, alpha-4 subunit of VLA-4 receptor, ITGA4, ITGA-IV|
|IgG2b, kappa, kappa|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.