CD51 (Integrin alpha V), clone: RMV-7, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-0512-85
Additional Details : Weight : 0.01000kg
DescriptionDescription: The RMV-7 monoclonal antibody reacts with the mouse CD51 molecule, the integrin alpha v chain. This approximately 120kDa surface molecule non-covalently associates with the beta subunits of the integrin family including beta3 (CD61), beta1 (CD29), beta5 and beta6 to form receptors for extracellular matrix components. Heterodimers of CD51/CD61 are expressed by platelets, T cells and granulocytes and mediate adhesion to fibrinogen, fibronectin, vitronectin and thrombospondin.Applications Reported: The RMV-7 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and functional assays. Soluble RMV-7 blocks adhesion to fibronectin and vitronectin and immobilized RMV-7 induces activation. (Please use Functional Grade purified RMV-7, cat. 16-0512, in functional assays.).Applications Tested: The RMV-7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|CD51 (Integrin alpha V)|
|PBS with 0.09% sodium azide; pH 7.2|
|Vitronectin Receptor alpha chain, VNR|
|Flow CytometryþFunctional AssayþImmunoprecipitationþNeutralization|
|IgG1, kappa, kappa|
For Research Use Only.