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CD54 (ICAM-1), Functional Grade, clone: eBioKAT-1 (KAT-1, KAT1), eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-0542-81

Additional Details : Weight : 0.01000kg

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Product Code. 15258477

  • 850.00 SEK / 50µg


Estimated Shipment date
from Supplier 29-10-2018
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Description

Description

Description: The eBioKAT-1 monoclonal antibody reacts with mouse CD54 (ICAM-1), which is a 95kDa member of the immunoglobulin superfamily. CD54 is expressed at low levels on leukocytes and high endothelial venules, and expression increases in response to inflammatory cytokines. ICAM-1 binds to LFA-1 and this interaction is required for the transendothelial migration of T cells. ICAM-1-deficient mice are viable, however the migration of leukocytes to sites of inflammation is reduced leading to impaired immune and inflammatory responses. Based on the regulated expression of ICAM-1, it has been suggested that ICAM-1 may increase leukocyte extravasation at sites of inflammation, whereas the constitutively high expression of ICAM-2 mediates leukocyte traffic into non-inflamed tissue. The eBioKAT-1 monoclonal antibody recognizes a different epitope than the YN1/1.7.4 monoclonal antibody.Applications Reported: This eBioKAT-1 (KAT-1, KAT1) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections. The KAT-1 monoclonal antibody has also been reported to inhibit the in vivo ICAM-1-mediated migration of leukocytes.Applications Tested: This eBioKAT-1 (KAT-1, KAT1) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD54 (ICAM-1)
Monoclonal
1mg/mL
PBS with no preservative; pH 7.2
P13597
BB2, P3.58, ICAM-1, cell surface glycoprotein P3.58, intercellular adhesion molecule 1, human rhinovirus receptor, major group rhinovirus receptor
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Immunoprecipitation, Neutralization
eBioKAT-1 (KAT-1, KAT1)
Functional Grade
Icam1
Liquid
Icam1
IgG2a, kappa, kappa
50μg
Store at 2-8°C.
Primary
15894
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