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CD55, PE, clone: 143-30, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-0559-41

Additional Details : Weight : 0.23750kg

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Product Code. 15506796

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on +46 31 352 32 00 for assistance



Description: The 143-30 monoclonal antibody reacts with human CD55, the 64-73kDa molecule also known as DAF (Decay-Accelerating Factor). CD55 is a GPI-anchored single chain glycoprotein expressed on all cells in contact with serum including all hematopoietic cells and vascular endothelium; a soluble form of CD55 is also present in plasma and body fluids. CD55 is thought to play a role in the protection of cells from the damage by autologous complement via prevention of the amplification steps of the complement cascade. Antibodies against CD55 are mitogenic in the presence of phorbol esters.Applications Reported: The 143-30 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 143-30 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.


25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Flow Cytometry
5μL (0.25μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Decay accelerating factor, DAF
IgG1, kappa

For Research Use Only.