CD6 Monoclonal Antibody (IM348), eBioscience™, Invitrogen™

Description

Description: The monoclonal antibody IM348 recognizes mouse CD6, a transmembrane glycoprotein of the scavenger receptor family. The extracellular domain contains three scavenger receptor cysteine rich (SRCR) domains, a transmembrane region and a cytoplasmic domain of variable length depending on the isoform. CD6 is expressed on most T lymphocytes. Expression between human and mouse is slightly different. In mice B cells in the spleen do not have CD6, while humans express it on a subset of B lymphocytes. The CD6 ligand is CD166, also known as Activated Leukocyte Cell Adhesion Molecule (ALCAM). Through interactions with CD166 or homotypic CD6-CD6, a role exists in adhesion and migration during organogenesis. The intracellular domain of CD6 is constitutively phosphorylated, suggesting a role in T cell activation and signaling. In humans depletion of CD6-expressing cells increases graft versus host tolerance in transplants. Applications Reported: This IM348 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistology staining of frozen tissue sections. Applications Tested: This IM348 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL.

CD6, also known as T12, is a member of the scavenger receptor superfamily found on T and B cell subsets, thymocytes, and acute lymphocytic leukemia cells (ALL). CD6 interacts with its ligand CD166/ALCAM (activated leukocyte cell adhesion molecule) and serves as a coreceptor for T cell activation and stabilizer of the immunological synapse. CD6-ALCAM mediated cell adhesion is also important for T cell proliferation. CD6 may exert some its functions via association with CD5, probably by fine-tuning CD5 signaling. Ligation of CD6 has antiapoptotic role in chronic lymphocytic leukemia B cells.