CD69, clone: H1.2F3, eBioscience™
Armenian Hamster Monoclonal Antibody
Brand: Affymetrix eBioscience 14-0691-82
Additional Details : Weight : 0.01000kg
DescriptionDescription: The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation.Applications Reported: The H1.2F3 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. H1.2F3 has also been reported in in vitro cell activation. (Please use Functional Grade purified H1.2F3, cat. 16-0691, in functional assays.).Applications Tested: The H1.2F3 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|AIM, BL-AC/P26, CLEC2C, EA1, GP32/28, MLR-3, C-type lectin domain family 2, member C, CD69 antigen|
|Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation|
|Store at 2-8°C.|
For Research Use Only.