Invitrogen™ CD8 alpha Monoclonal Antibody (5H10), Pacific Orange™

Beskrivning

The 5H10 monoclonal antibody (mAb) recognizes the mouse CD8aplpha chain. 5H10 mAb has been used in other labs for flow cytometry and inhibition of T cell responses to IL-2. Every lot is tested by flow cytometry using freshly harvested mouse splenocytes. If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted. Cells should be analyzed within 18 hours of staining for best results. For Pacific Orange™ conjugates: excitation 405 nm/peak emission 551 nm. Matching isotype control is available: Rt IgG2b Pacific Orange™. Use a 575 or 585 nm band pass filter for optimal brightness from Pacific Orange™ dye.

CD8, also known as cluster of differentiation 8, is a type I transmembrane glycoprotein of the immunoglobulin family that plays a crucial role in T cell differentiation, activation, and signal transduction. It is expressed as either a heterodimer (CD8 alpha beta) or a homodimer (CD8 alpha alpha). The CD8 alpha beta form is predominantly found on the majority of thymocytes and a subpopulation of mature alpha beta TCR T cells, while the CD8 alpha alpha form is expressed on gamma delta TCR T cells, a subset of intestinal intraepithelial lymphocytes (IELs), and dendritic cells. CD8 functions as a co-receptor for major histocompatibility complex class I (MHC-I) molecules, working alongside the T cell receptor (TCR). The CD8 alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes, and dendritic cells as disulfide-linked homodimers of CD8 alpha. Upon ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), CD8 recruits lymphocyte-specific protein tyrosine kinase (Lck), leading to lymphokine production, increased motility, and activation of cytotoxic T lymphocytes (CTLs). Activated CTLs are vital for clearing pathogens and tumor cells. The differentiation of naive CD8+ T cells into CTLs is strongly enhanced by cytokines such as IL-2, IL-12, and TGF-beta1. Through its interactions with MHC-I and association with protein tyrosine kinase p56lck, CD8 plays a significant role in T cell development and the activation of mature T cells.