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CD86 (B7-2) Mouse anti-Human, PE-Cyanine5, Clone: IT2.2, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 15-0869-42

Additional Details : Weight : 0.23750kg

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Product Code. 15507746

  • 2460.00 SEK / 100 tests


EU Stock 1
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Description

Description

Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered. CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.
Specifications

Specifications

CD86 (B7-2)
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P42081
B7-2, B7.2, B70, CD28LG2, LAB72, MGC34413, B-lymphocyte activation antigen B7-2, B-lymphocyte antigen B7-2, B7-2 antigen, BU63, CD28 antigen ligand 2
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
IT2.2
PE-Cyanine5
CD86
Liquid
CD86
IgG2b, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
942
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