CD8a, Alexa Fluor 700, clone: RPA-T8, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 56-0088-42
Additional Details : Weight : 0.23750kg
DescriptionDescription: The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.Applications Reported: This RPA-T8 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RPA-T8 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|CD8, Leu-2, MAL, p32, CD8a|
|Alexa Fluor 700|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.