CD8a, Biotin, clone: 53-6.7, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 13-0081-81
Additional Details : Weight : 0.25000kg
DescriptionDescription: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.Applications Reported: The 53-6.7 antibody has been reported for use in flow cytometric analysis and immunohistochemistry.Applications Tested: The 53-6.7 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|CD8, Leu-2, MAL, p32, CD8a|
|Flow CytometryþImmunohistochemistry (Frozen)|
|IgG2a, kappa, kappa|
|Store at 2-8°C. Do not freeze.|
For Research Use Only.