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CD90 (Thy-1) Mouse anti-Human, APC-eFluor 780, Clone: eBio5E10 (5E10), Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 47-0909-42

Additional Details : Weight : 0.01000kg

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Product Code. 16606364

  • 3740.00 SEK / 100 tests
Estimated Shipment: 18-08-2022
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Description

Description

Description: The eBio5E10 monoclonal antibody reacts with human CD90, also known as Thy-1 (thymus cell antigen-1). CD90 is a 25-35 kD receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, a small subset of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is expressed on a subset of immature, CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+. The CD90+CD34+ population is enriched for cells capable of long-term culture. CD90 is involved in regulation of adhesion and signal transduction by T cells. Applications Reported: This eBio5E10 (5E10) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio5E10 (5E10) antibody has been pre-diluted and tested by flow cytometric analysis of human erythroleukemia (HEL) cells. This may be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633-647 nm). Please make sure that your instrument is capable of detecting this fluorochrome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser

CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90 is expressed in murine T cells, thymocytes, neural cells, Kupffer′s cells and fibroblasts. CD90 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of CD90 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor. Diseases associated with CD90 dysfunction include nasopharyngeal carcinoma and thymoma.

Specifications

Specifications

CD90 (Thy-1)
Monoclonal
5 μL/Test
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
P04216
Thy1
Affinity chromatography
RUO
7070
4°C, store in dark, DO NOT FREEZE!
Liquid
Flow Cytometry
eBio5E10 (5E10)
APC-eFluor 780
Thy1
CD7; CD90; CDw90; FLJ33325; LOC100339279; T25; Thy 1.2; Thy1; Thy-1; Thy-1 antigen; Thy-1 cell surface antigen; thy-1 glycoprotein; Thy-1 membrane glycoprotein; Thy-1 protein; Thy-1 T-cell antigen; Thy1.1; Thy1.2; Thy-1.2; thymus cell antigen 1, theta; Thymus cell surface antigen
Mouse
100 Tests
Primary
Human
Antibody
IgG1 κ
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