CD95 (APO-1/Fas), clone: DX2, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-0959-80
Additional Details : Weight : 0.01000kg
DescriptionDescription: The DX2 monoclonal antibody reacts with human CD95 (Fas, Apo-1), a 40-50kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. DX2 does not block binding of EOS9.1, another antibody specific for human CD95.Applications Reported: The DX2 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. DX2 has also been reported in in vitro studies of Fas-induced apoptosis. EOS9.1 is recommended for induction of apoptosis in vitro .Applications Tested: The DX2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|ALPS1A, APO-1, APT1, Apo-1 Fas, CD95, FAS1, FASTM, TNFRSF6|
|Flow Cytometry, Functional Assay, Immunohistochemistry|
|IgG1, kappa, kappa|
|Store at 2-8°C.|
For Research Use Only.