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Eurofins DiscoverX Cdk3/Cyclin E Protein, Active

Produktkod. 17300244
Encompass_Preferred
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Kvantitet:
10 μg
250 μg
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17300244 10 μg
17310244 250 μg
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Produktkod. 17300244 Leverantör Eurofins DiscoverX Leverantörsnummer 14487

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Active complex of recombinant full length human Cdk3 containing a C-terminal His6-tag, and recombinant full-length human Cyclin E containing an N-terminal GST-tag, for use in Kinase Assays.

The cyclin-dependent kinases (CDKs), a subfamily of protein kinases, promote cell cycle progression. In mammals, nine different CDKs (cdk 1 to cdk 9; cdk 1 is better known as cdc 2) have been identified. CDKs are regulated on various levels including binding of proteins (cyclins, inhibitors, and assembly factors), protein degradation, transcriptional control, localization, and multiple phosphorylations. This leads to timely regulation of CDK activity, allowing progression from one cell cycle phase to the next. Cdk3 participates in the G1–S progression at least partially by binding to E2F-1, E2F-2, or E2F-3 through DP-1 and by enhancing their transcriptional activities.

GenBank X66357 / GenBank NM_001238

Specifikationer

Formulering mg/mL of enzyme in 50 mM Tris/HCl pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Molekylvikt (g/mol) 36 kDa (CDK3) and 74 kDa (Cyclin E)
Kvantitet 10 μg
Art Human
Rekombinant Recombinant
Protein Tag C-terminal 6His / N-terminal GST
Uttryckssystem Recombinant enzyme expressed in Sf21 insect cells
Proteinform Full-length
Renhet eller kvalitetsklass ≥58%
Protein CDK3/Cyclin E, active
Produkttitel
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