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CDK9/Cyclin K Recombinant Human Protein

CDK9/Cyclin K Recombinant Human Protein

Brand:  Invitrogen PR7510A

Additional Details : Weight : 0.01000kg

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Product Code. 15468603

  • 20540.00 SEK / 100µg


Estimated Shipment date
from Supplier 05-12-2017
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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Description

Description

CDK9 is a serine⁄threonine kinase that phosphorylates the c-terminal domain of the largest subunit of RNA polymerase II and is involved in cell cycle regulation, This protein is coexpressed and copurified with CyclinK.
Specifications

Specifications

NP_001252, NP_003849
Protein assays and analysis
Frozen
CDK9/Cyclin K Recombinant Human Protein
100μg
For research use only. Not for use in diagnostic procedures
CDK9/cyclin K, TAK
CDK9/cyclin K was pre-diluted in enzyme dilution buffer and assayed in 25mM MOPS (pH 7.2), 10mM MgCl2, 0.5mM EGTA, 0.5mM Na3VO4, 5mM β-glycerophosphate, 2.5mM DTT, 0.01% Triton™ X-100, 100μM ATP, 200μg/mL CHKtide substrate peptide (KKKVSRSGLYRSPSMPENLNRPR) and trace [32P]-γ-ATP for 10 minutes at 30°C
Baculovirus
CDK9/Cyclin K Recombinant Human Protein
Human
0.18mg/mL
Frozen
91.8kDa
70% by SDS-PAGE
–80°C
1 Each
CDK9/Cyclin K Recombinant Human Protein
Kinase assay
CDK and CHK
2.4nmoles of phosphate transferred to CHKtide substrate peptide (KKKVSRSGLYRSPSMPENLNRPR) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 5μg/mL
His Tag
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