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Invitrogen™ CellBlox™ Blocking Buffer, 100 Tests

Formulated to block nonspecific binding of Invitrogen™ NovaFluor™ labels with cells, which can result in higher background labeling. Also recommended for cyanine-based dyes or cyanine-based tandem dyes to block non-specific interactions with monocytes and macrophages to minimize background labeling.

Brand:  Invitrogen™ B001T03F01

Produktkod. 17263675

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Beskrivning

Beskrivning

  • Formulated to block nonspecific binding of Invitrogen™ NovaFluor™ labels with cells that can result in higher background labeling
  • CellBlox™ Blocking Buffer is a non-antibody, non-protein–based blocking solution, and should be used every time a NovaFluor dye is used for labeling any cell type to minimize background labeling
  • Recommended for use with cyanine-based dyes or cyanine-based tandem dyes to block non-specific interactions with monocytes and macrophages to minimize background labeling
  • Use of CellBlox Blocking Buffer requires minimal change to most flow cytometry staining protocols
  • Add 5 μL of buffer directly to a cell suspension containing 103 to 108 cells prior to the addition of an antibody, with 100 μL as a final staining volume
  • Buffer may be added to an antibody cocktail mixture prior to labeling cells, by adding 5 μL of buffer for every stained sample to be labeled with the antibody cocktail mixture, with 100 μL as a final staining volume
  • Always use CellBlox Blocking Buffer with NovaFluor dyes when labeling cells for best background reduction
  • Compatible with all fluorophores and with Invitrogen™ LIVE/DEAD™ Fixable Dead Cell Stains
  • Can be used with any fluorophore-antibody conjugate as a high-performance monocyte and macrophage blocking solution
  • CellBlox Blocking Buffer is compatible with other blocking reagents such as Fc Block, blocking proteins, Brilliant Stain Buffer, and Super Bright Complete Staining Buffer
  • Buffer is not required when labeling antibody-capture beads
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Invitrogen™ CellBlox™ Blocking Buffer, 100 Tests > 100 tests

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