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Invitrogen™ CellTrace™ Violet Cell Proliferation Kit, for flow cytometry

CellTrace™ Violet Cell Proliferation Kit, for flow cytometry

Brand:  Invitrogen™ C34571

684.60 SEK valid until 2020-12-18
Use promo code "14718" to get your promotional price.

Additional Details : Weight : 0.50000kg

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Product Code. 15579992

  • 978.00 SEK / 1 set

In stock, ready to ship: 38
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CellTrace™ Violet Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry.

  • Superior performance—bright, single-peak staining enables visualization of multiple generations
  • Long-term signal stability—well-retained in cells for several days post stain
  • Versatile—multiple colors available to easily combine with antibodies or markers of cell function, such as GFP
  • Simple, robust staining protocol

    View a selection guide for all CellTrace™ Cell Proliferation Kits for flow cytometry.

    Superior fluorescent staining
    Successful proliferation analysis by dye dilution (see figure below) requires an extremely bright dye to distinguish fluorescently labeled cells from auto-fluorescence after several cell divisions. The intense fluorescent staining provided by CellTrace™ Violet dye enables the visualization of ten or more generations of proliferating cells before the signal is overwhelmed by intrinsic cellular auto-fluorescence. Consistent, homogeneous staining results in very little fluorescence variation between cells in a population, so distinct generations can be seen without any requirement for complex modeling software.

    Long-term signal retention
    Unlike stains that label the lipid membrane of cells, CellTrace™ Violet stain easily crosses the plasma membrane and covalently binds inside cells where the stable, well-retained fluorescent dye provides a consistent signal, even after several days in a cell culture environment. CellTrace™ Violet dye binds covalently to all free amines on the surface and inside of cells and shows little cytotoxicity, with minimal observed effect on the proliferative ability or biology of cells.

    Easy multiplexing with other fluorophores
    The violet excitation and narrow emission of CellTrace™ Violet dye make it ideal for multiplexing due to the limited spectral overlap with other common dyes (Alexa Fluor™ 488, FITC, and RPE) and fluorescent proteins (Green Fluorescent Protein (GFP) and mCherry) (see Fluorescence Spectra for CellTrace™ Violet stain below).

    Simple, robust staining protocol
    The CellTrace™ Violet Cell Proliferation Kit contains convenient single-use vials of dry dye to permit small-scale experiments without preparing excess quantities of dye. A stock solution is prepared by dissolving the contents of a vial in anhydrous DMSO prior to use. To stain 1 mL of cells in protein-free medium, 1μL of this stock solution is typically used. Cells should be stained for 20 minutes at room temperature with gentle agitation. A brief wash with complete medium will then quench any dye remaining in solution.
  • Specifications


    Contains 1 vial of CellTrace™ Violet reagent and 1 vial of DMSO (100 µL).
    Store in freezer (-5 to -30°C) and protect from light.
    Flow Cytometer
    20 Reactions
    CFSE & Related Compounds
    CellTrace™ Violet
    DMSO (Dimethylsulfoxide)
    1 Kit(s)
    Room Temperature
    Contains 1 vial of CellTrace™ Violet reagent and 1 vial of DMSO (100μL)

    For Research Use Only. Not for use in diagnostic procedures.