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DLL4 (delta-like 4), Functional Grade, clone: HMD4-1, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-5948-82

Additional Details : Weight : 0.01000kg

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Product Code. 15248707

  • 1106.00 SEK / 100µg


Estimated Shipment date
from Supplier 21-11-2018
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Description

Description

Description: This HMD4-1 monoclonal antibody reacts with the extracellular domain of mouse Delta-like 4, one of five type I transmembrane proteins that serves as a Notch receptor ligand. Upon binding the Notch receptor (e.g., Notch 1-4), Delta-like 4 undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase. The second cleavage event releases the Notch intracellular domain (NICD), which subsequently translocates into the nucleus, heterodimerizes with the DNA-binding protein RBP-J, recruits co-activator molecules, and ultimately activates transcription. Delta-like 4 is expressed by thymic lymphoid and stromal cells, as well as macrophages and dendritic cells in the spleen. This molecule is also expressed in non-immune cells, including vascular smooth muscle and endothelial cells. Delta-like 4 plays a role in T cell development, regulation of experimental autoimmune encephalomyelitis, and the formation of mature vasculature.The HMD4-1 monoclonal antibody has been reported to have blocking activity and crossreact to rat.Applications Reported: The HMD4-1 antibody has been reported for use in functional assays.Applications Tested: This HMD4-1 antibody has been tested by flow cytometric analysis of mouse Delta-like 4-transfected cells. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

DLL4 (delta-like 4)
Monoclonal
1mg/mL
PBS with no preservative; pH 7.2
Q9JI71, D3ZHH1
MGC126344, hdelta2
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Murine, Rat
Functional Assay
HMD4-1
Functional Grade
Dll4
Liquid
Dll4
IgG
100μg
4° C
Primary
311332, 54485
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