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Dendritic Cell Marker DCIR2 Rat anti-Mouse, Clone: 33D1, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-5884-82

Additional Details : Weight : 0.01000kg

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Product Code. 15227557

  • 828.00 SEK / 100µg

Estimated Shipment: 29-06-2021

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Description

Description

Description: The 33D1 monoclonal antibody reacts with a mouse dendritic cell (DC)-specific surface marker, DCIR2. The nature and biological activity of DCIR2 is unknown. DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer′s patch. Bone marrow dendritic cells require GM-CSF to express DCIR2, and this expression is downregulated in the presence of IL-4. DCIR2 has been detected in vivo in brain dendritic cells post infection with Toxoplasma gondii. Applications Reported: This 33D1 antibody has been reported for use in flow cytometric analysis, immunohistology staining of frozen tissue sections, and immunohistochemical staining. It has also been reported in complement-mediated cytotoxicity. Applications Tested: The 33D1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. For detection of purified 33D1 it is recommended not to use goat anti-rat due to its low affinity for 33D1. Rather, we recommend using biotin mouse anti-rat (cat. 13-4813).

Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. The nature and biological activity of DCIR2 is unknown. DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer′s patch. Bone marrow dendritic cells require GM-CSF to express DCIR2, and this expression is downregulated in the presence of IL-4. DCIR2 has been detected in vivo in brain dendritic cells post infection with Toxoplasma gondii.
Specifications

Specifications

Dendritic Cell Marker DCIR2
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
Liquid
Clec4a4
IgG2b, kappa
100 μg
4° C
Primary
474145
Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen)
33D1
Unconjugated
Clec4a4
DC Marker; DC inhibitor receptor 2; DCIR2
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
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