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Diethyl Pyrocarbonate, MP Biomedicals™

Catalog Number 11416672
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Quantity:
100 mL
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Description

Used to inactivate RNase enzymes. Diethyl Pyrocarbonate, MP Biomedicals is sensitive to moisture and pH. DEPC is also sensitive to ammonia, which causes decomposition to urethane, a possible carcinogen. It protects RNA against degradation and is effective

DEPC inactivates RNase in solution at about 0.1% (v/v). It inhibits the ryanodine binding to ryanodine/Ca2+ receptor channels in skeletal muscle in a dose- and time-dependent manner and increases the Ca2+ permeability of SR vesicles. DEPC is effective as a nuclease inhibitor with a variety of uses:

  • Modification reagent for His and Tyr residues in proteins
  • Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases
  • Used to modify proteins and nucleic acids
  • PCR reaction for treating deionized water, which reduces the risk of RNA being degraded by RNases
  • Dot blot hybridization, to dilute total RNA isolated from different micro-organisms
CAS 1609-47-8
Density 1.101g/mL at 25°C (Lit.)
Boiling Point 93°C to 94°C (18 mmHg)(literature)
Molecular Formula C6H10O5
Refractive Index n20/D 1.398 (Lit.)
MDL Number MFCD00009106
Quantity 100 mL
Synonym diethyl pyrocarbonate, diethyl dicarbonate, depc, diethyl oxydiformate, baycovin, diethylpyrocarbonate, ethyl pyrocarbonate, dicarbonic acid, diethyl ester, dicarbonic acid diethyl ester, diethyl pyrocarbonic acid
Solubility Information Soluble in alcohols, esters, ketones, hydrocarbons. A 50% w/w solution in ethanol may be prepared. Slowly soluble in water with hydrolytic decomposition yielding ethanol and CO2.
InChI Key FFYPMLJYZAEMQB-UHFFFAOYSA-N
SMILES CCOC(=O)OC(=O)OCC
IUPAC Name ethoxycarbonyl ethyl carbonate
Molecular Weight (g/mol) 162.14
PubChem CID 3051
ChEBI CHEBI:59051
Formula Weight 162.10
Concentration or Composition (by Analyte or Components) Working Concentration: For Treatment of water to inhibit Rnase: Add 1mL DEPC to 1L water. Let sit overnight. Autoclave.
Physical Form Liquid
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Safety and Handling

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Product Identifier
  • Diethyl Pyrocarbonate
 Signal Word
  • Warning
 Hazard Category
  • Acute toxicity Category 4
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H335-May cause respiratory irritation.
 Precautionary Statement
  • P101-If medical advice is needed, have product container or label at hand.
  • P102-Keep out of reach of children.
  • P103-Read label before use.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : diethyl pyrocarbonate
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