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Gibco™ DMEM/F-12
DMEM/F-12
Brand: Gibco™ 11320074
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Description
Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Gibco™ DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.Gibco™ DMEM / F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components.Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco™ DMEM / F-12 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco™ DMEM / F-12 product made in our Grand Island facility (11320-033). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
DMEM / F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM / F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM / F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Specifications
ISO 13485 standards, FDA | |
Dulbecco's Modified Eagle Medium, 500mL, Liquid Form, 1 X Concentration, 7 to 7.4 pH, 2 to 8°C Storage Temperature, 12 Months Shelf Life, Certified to ISO 13485 Standards, For Supporting Growth of Mammalian Cells | |
Liquid | |
Glutamine, Phenol Red, Sodium Pyruvate, Sodium Bicarbonate | |
For mammalian cell culture | |
500 mL |
2 to 8°C | |
1 X | |
Dulbecco's Modified Eagle Medium | |
No HEPES | |
7 to 7.4 | |
CE-IVD |