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Gibco™ DMEM, high glucose, GlutaMAX™ Supplement, pyruvate

DMEM, high glucose, GlutaMAX™ Supplement, pyruvate

Brand:  Gibco™ 31966047

Additional Details : Weight : 0.50000kg

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Product Code. 12077549

  • 1828.00 SEK / 5000mL

In stock, ready to ship: 174

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For Research Use Only. All usage must comply with product instructions.


Description

Description

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Product use
For human ex vivo tissue and cell culture processing applications. CAUTION: When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco™ DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system
DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical DMEM product made in our Scotland facility (31966-021). This facility is also registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standard.
Specifications

Specifications

1 x
With - High glucose, GlutaMAX™, phenol red, without - Sodium pyruvate, HEPES
2 to 8°C
12 Months
Liquid
Liquid
6.8 to 7.2
Tissue and cell culture processing, mammalian cell culture
Dulbecco's Modified Eagle Medium (DMEM), 10 x 500mL, Liquid, 1X Concentration, 6.8 to 7.2 pH, With High Glucose, GlutaMAX™, Sodium Pyruvate, Phenol Red, 12 Months Shelf Life, 320 to 360mOsm/kg Osmolality, For Mammalian Cell Culture
Dulbecco's Modified Eagle Medium
2 to 8°C
10 x 500mL
ISO 13485 standard
High glucose, sodium pyruvate, GlutaMAX™, phenol red
HEPES
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