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Invitrogen™ DNA Polymerase I, Large (Klenow) Fragment

Codice prodotto. 10023042
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2 x 500 Unit(s)
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Codice prodotto. Quantity Dimensione della confezione
10023042 2 x 500 Unit(s) Each
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For Research Use Only. All usage must comply with product instructions.
Codice prodotto. 10023042 Fornitore Invitrogen™ N. del fornitore 18012096
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Beräknad leverans: 27-11-2026
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Les retours ne sont pas autorisés pour ce produit. Consulta la politica di reso

For Research Use Only. All usage must comply with product instructions.

Descrizione

DNA Polymerase I, Large (Klenow) Fragment

DNA Polymerase I, Large (Klenow) Fragment is a DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I, but does exhibit the 5' to 3' DNA polymerase and 3' to 5' exonuclease activities.

Applications:
Fill-in of 5´ overhangs (1). Synthesis of probes by random primers labeling method (2). Sequencing single- and double-stranded DNA (3). Site-directed mutagenesis.

Source:
Purified from E. coli expressing the Klenow fragment on a plasmid.

Performance and Quality Testing:
SDS-PAGE purity; single-stranded and double-stranded endodeoxyribonuclease and self-priming assays; performance evaluated in fill-in reaction.

Unit Definition:
One unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template
  • primer.
    • TRUSTED_SUSTAINABILITY

    Specifica

    Content And Storage –20°C
    Description DNA Polymerase I Large (Klenow) Fragment
    Quantity 2 x 500 Unit(s)
    For Use With (Application) Genotyping, Cloning
    Includes DNA polymerase I, large (Klenow) fragment is supplied with a vial of 10X REact™ 2 buffer [500mM Tris HCl (pH 8.0), 100mM MgCl2, 500mM NaCl], vial of dilution buffer

    For Research Use Only. Not for use in diagnostic procedures.

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