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Beskrivning
Konventionella restriktionsendonukleaser är en stor samling av högkvalitativa restriktionsenzymer, optimerade för att fungera i en av buffertarna i Five Buffer System. Dessutom tillhandahålls den universella Tango-bufferten för bekvämlighet vid dubbelrötning. Alla enzymer uppvisar 100 % aktivitet i de rekommenderade buffert- och reaktionsförhållandena. För att säkerställa konsekvent prestanda, Thermo Scientific restriktionsenzymreaktionsbuffertar innehåller förblandad BSA, vilket förbättrar stabiliteten hos många enzymer och binder föroreningar som kan finnas i DNA-beredningar.
| 5’ | G | Am6↓ | T | C | 3’ | ||||
| 3’ | C | T ↑ | Am6 | G | 5’ |
Thermo Scientific DpnI restriction enzyme recognizes Gm6A^TC sites and cuts best at 37°C in Tango buffer (isoschizomers: MalI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.
In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Features
- Superior quality—stringent quality control and industry leading manufacturing process
- Convenient color-coded Five Buffer System
- Includes universal Tango buffer for double-digestions
- BSA premixed in reaction buffers
- Wide selection of restriction endonuclease specificities
Applications
- Molecular cloning
- Restriction site mapping
- Genotyping
- Southern blotting
- Restriction fragment length polymorphism (RFLP)
- SNP
Note: DpnI requires the presence of N6-methyladenine within the recognition sequence to cleave DNA. DNA purified from a dam+ strain will be a substrate for DpnI. DpnI will only cleave fully-adenomethylated dam sites. Hemi-adenomethylated dam sites DpnI cleaves 60X more slowly. DpnI, Bsp143I, and MboI all recognize the same sequence, but have different methylation sensitivities and cleavage sites. Assayed using pBR322 DNA. For methylation sensitivity, refer to product specifications.
Specifikationer
Specifikationer
| Koncentration | 10 U/μL |
| Metyleringskänslighet | Dam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive |
| Enzym | Dpn I |
| Kompatibel buffert | 10X Buffer Tango |
| Känslig för värmeinaktivering | Yes |
| Optimal reaktionstemperatur | 37°C |
| Typ IIS RE | No |
| Kvantitet | 500 units |
| Produkttyp | Restriction Enzyme |
| Forskningskategori | Traditional Cloning |
Endast för forskning. Ej för diagnostik.
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