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Invitrogen™ Dynabeads™ Sheep anti-Rat IgG

Codice prodotto. 10592405
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5 mL
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2mL
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10592405 5 mL Each
30275709 2 mL 2mL
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For Research Use Only. All usage must comply with product instructions.

Codice prodotto. 10592405 Fornitore Invitrogen™ N. del fornitore 11035
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Les retours ne sont pas autorisés pour ce produit. Consulta la politica di reso

For Research Use Only. All usage must comply with product instructions.

The product can be used to isolate any cell from any species (except rat), depending on the specificity of the monoclonal rat IgG employed.

Dynabeads Sheep Anti-Rat IgG are 4.5 μm superparamagnetic beads with affinity purified polyclonal sheep anti-rat IgG covalently bound to the bead surface. These beads can be used to isolate any cell from any species (except rat), depending on the specificity of the monoclonal rat IgG employed. Dynabeads Sheep Anti-Rat IgG are especially well suited for cell isolation from mouse samples (cross-reactivity to mouse antibodies is high and cross-reactivity to human antibodies is minimal).

Advantages of Dynabeads Sheep Anti-Rat IgG:

  • Works with any cell type, from any sample and from any species (except rat)
  • Fast and efficient depletion or positive isolation of any target cell for molecular downstream assays using rat primary antibody
  • Obtain pure and untouched cells using a mixture of rat IgGs to deplete unwanted cells

Magnetic bead-based separation offers easy handling

The rat primary antibody may be added to the cell sample (indirect technique) or pre-coated onto the Dynabeads Sheep Anti-Rat IgG (direct technique). Regardless of which technique is used, when the cell sample and the Dynabeads Sheep Anti-Rat IgG are mixed, the beads bind to the target cells. Placing the sample on a magnet separates the bead-bound cells from the rest of the sample. One or more rat primary antibodies may be used to either deplete the sample of unwanted cells (negative isolation) or to capture specific cells for molecular downstream experiments (positive isolation). For positive isolation of bead-free cells (e.g., for flow cytometry), we recommend using your rat antibody in combination with the Dynabeads FlowComp Flexi Kit.

Useful for many sample types

Using Dynabeads Sheep Anti-Rat IgG, you can isolate cells from many starting samples including whole blood, mouse spleen or lymph node cells, and tissue digests.

Learn more about Dynabeads products

Información de órdenes

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Especificaciones

Antibody Fragment Whole Antibody
Cell Type All cells from all species, except rat
Clonality Polyclonal
Content And Storage This product contains 5 mL of Dynabeads™ Sheep Anti-Rat IgG (4 x 108 Dynabeads™/mL) in phosphate buffered saline (PBS), pH 7.4, containing 0.1% bovine serum albumin (BSA) and 0.02% sodium azide.

Store at 2°C to 8°C.
Product Type Antibody Coated Bead
Dye Type Dynabeads™
Host Species Sheep
Includes 5 mL of Dynabeads Sheep Anti-Rat IgG (4 x 108 Dynabeads/mL) in phosphate buffered saline (PBS), pH 7.4, containing 0.1% bovine serum albumin (BSA) and 0.02% sodium azide as a preservative.
Isolation Technology Depletion, negative isolation or positive isolation
No. of Cells Processes ∼2 x 109 cells total
Output Viability >95%
Product Line DYNAL, Dynabeads
Purity or Quality Grade Research Grade
Quantity 5 mL
Reactivity All species, except rat
Sample Type PBMC, Tissue Digests, Blood
Shipping Condition Room Temperature
Starting Material Cell No. 1 x 107 PBMCs per isolation
Target Species All species, except rat
Diameter (Metric) 4.5 μm
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For Research Use Only. Not for use in diagnostic procedures.

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