Invitrogen™ EAPP Polyclonal Antibody

Beskrivning

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Positive Control - WB: human placenta tissue, human Hela whole cell, human COLO-320 whole cell, human Jurkat whole cell, human HEK293 whole cell, human K562 whole cell. IHC: human rectal cancer tissue, human rectal cancer tissue, human rectal cancer tissue, mouse cardiac muscle tissue. ICC/IF: Hela cell. Flow: HepG2 cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

This protein may play an important role in the fine-tuning of both major E2F1 activities, the regulation of the cell-cycle and the induction of apoptosis. Promotes S-phase entry, and inhibits p14(ARP) expression. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The C-terminal of EAPP binds the N-terminal of E2F1 and also interacts with E2F2 and E2F3, but not E2F4.