Thermo Scientific™ Easy-Titer™ Human IgG (H+L) Assay Kit

Highlights:

• Kits for five popular targets – choose a kit specific for a particular species and class of immunoglobulin; no cross-reaction with other species and classes of the target antibody

• Antibody-based specificity – measure concentration of target antibody in a sample, not just total protein; no need to purify antibody to assess its concentration

• Faster and easier than ELISA – three-component, homogenous assay; 10 minutes total incubation time

• No special equipment needed – uses standard vortex mixer, pipetter, 96-well microplate, plate shaker and reader (measure absorbance at 340nm or 400nm)

• Sensitive – assay range (standard curve) 8 to 500ng/mL; requires starting sample of at least 300ng/mL

• Reproducible – coefficient of variation less than 5%; error depends on dilution and pipetting technique

• Antibody standards sold separately – use any antibody standard with proper target identity and known concentration (greater than 10μg/mL)

Easy-Titer Antibody Assays are faster and easier than ELISA:

1. Prepare standards (5 to 500ng/mL) by diluting purified antibody in Kit Dilution Buffer

2. Prepare samples by diluting at least 20-fold in Dilution Buffer to within assay range (8 to 500ng/mL).

3. Vortex vial of microsphere beads to create homogeneous suspension.

4. Pipette 20μL of bead suspension and 20μL of each sample and standard into 96-well microplate wells.

5. Incubate microplate for 5 minutes with vigorous mixing.

6. Add 100μL of Kit Blocking Reagent.

7. Incubate microplate for 5 minutes with vigorous mixing.

8. Measure absorbance on standard plate reader (340nm or 405nm).

9. Plot standard curve and interpolate samples to determine concentration.

Includes:

Each kit contains target-specific IgG sensitized microspheres, dilution buffer and blocking buffer