Ephrin B1, clone: 25H11, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-5300-82
Additional Details : Weight : 0.01000kg
DescriptionDescription: The monoclonal antibody 25H11 recognizes mouse Ephrin B1, a 47kDa membrane protein. Ephrin B1 belongs to a family of ephrins composed of type A (linked to the cell membrane through a glycosylphosphatidyl inositol group) or type B (transmembrane) proteins. The type B ephrins consist of 3 members (B1, B2, and B3) each of which contains a cytoplasmic domain containing tyrosine residues that can be phosphorylated. Ephrin B and ephrin B receptors (Eph B) can interact to potentiate bidirectional downstream signaling between cells expressing either or both the ligand and receptor. Ephrins have been shown to play a role in neural crest cell development through both neurogenesis and neural migration, as well as development of kidney and heart cyto-architecture. Overexpression of type B ephrins in cancer cells is associated with increased vascularity and highly invasive tumors.Applications Reported: This 25H11 antibody has been reported for use in immunohistochemical staining of frozen tissue sections and immunoprecipitation.Applications Tested: This 25H11 antibody has been tested immunohistochemistry on frozen mouse kidney tissue sections at less than or equal to 10 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|ELK, EPHT2, FLJ37986, Hek6, NET|
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunoprecipitation|
|IgG2a, kappa, kappa|
|Store at 2-8°C.|
For Research Use Only.