Estrogen Receptor alpha Mouse anti-Human, Clone: tbykt, eBioscience™
Mouse Monoclonal Antibody
Marque: Affymetrix eBioscience 14-9740-82
Informations supplémentaires : Weight : 0.09500kg
DescriptionApplications Tested: This tbykt antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH or high pH antigen retrieval and can be used at less than or equal to 0.5 µg/mL. This tbykt antibody has been tested by immunocytochemistry of fixed and permeabilized human cells and can be used at less than or equal to 1.0 µg/mL. This tbykt antibody has been tested by western blot of reduced HeLa cell lysate and can be used at less than or equal to 5.0 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.
|Estrogen Receptor alpha|
|PBS with 0.09% sodium azide; pH 7.2|
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot|
For Research Use Only.