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Invitrogen™ Estrone-3-Sulfate (E1S) Competitive ELISA Kit
Competitive ELISA
Brand: Invitrogen™ EIA17E3S
Additional Details : Weight : 0.33820kg
Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, assay buffer, wash buffer, chromogen, stop solution, plate covers and lot-specific technical data sheet.
Description
The Human Estrone-3 Sulfate (E1S) ELISA quantitates human estrone-3 Sulfate in human serum,plasma,urine,dried fecal extracts or cell culture medium.
Principle of the method
The Estrone-3-Sulfate (E1S) Competitive ELISA research-use-only kit uses a specifically generated antibody to measure Estrone-3-Sulfate (E1S) in a variety of matrices,including serum,plasma,urine,and fecal samples. The kit will quantitatively measure E1S present in diluted buffer samples and tissue culture media samples. An E1S standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An E1S-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to E1S to each well. After a 2-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound E1S-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.
Specifications
40.96-4000 pg/mL | |
Estrone-3-Sulfate (E1S) Competitive ELISA Kit | |
HRP | |
ELISA Kit | |
Fecal Extract, Plasma, Serum, Supernatant, Urine | |
Human | |
Colorimetric Microplate Reader | |
4.2% | |
HRP | |
RUO | |
Fecal Extract,50 μL; Plasma, 1 μL; Serum, 1 μL; Supernatant, 50 μL; Urine,6.25 μL | |
Human | |
2 hr. 30 min. |
26.4 pg/mL | |
Multiplex assay on the Luminex Platform | |
Fluorescence | |
Steroid hormone | |
96 assays | |
ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti | |
8.5% | |
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
96 Tests | |
Reproductive Biology | |
-20°C | |
1 hr. 20 min. |