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Gibco™ ExpiFectamine™ 293 Transfection Kit
Designed for transient transfection of high density cultures of HEK (human embryonic kidney) 293 cells.
13170.00 SEK - 478200.00 SEK
Specifications
Cell Line | Expi293F™ |
---|---|
Classification | Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free |
Cell Type | Established Cell Lines |
Sample Type | Plasmid DNA |
Transfection Technique | Lipid-based Transfection |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
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Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
13395544
|
Gibco™
A14525 |
For 10 L of culture |
111600.00 SEK
10 L |
In Stock Log in to see stock. |
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13423269
|
Gibco™
A14526 |
For 50 L of culture |
478200.00 SEK
1 set |
In Stock Log in to see stock. |
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13385544
|
Gibco™
A14524 |
For 1 L of culture |
13170.00 SEK
1 L |
In Stock Log in to see stock. |
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Description
The ExpiFectamine™ 293 Transfection Kit is a core component of the Expi293™ Expression System. It is designed for transient transfection of high density cultures of HEK (human embryonic kidney) 293 cells. The high-efficiency, cationic, lipid-based transfection reagent and transfection enhancers are designed to power the highest possible level of protein expression from Expi293F™ cells cultured in Expi293™ Expression Medium
- Designed specifically for transfection of high density suspension cell culture, with matching transfection enhancers that boost transfection performance and protein expression
- Achieves protein yields 2- to 10-fold higher than other transfection reagents used on high density 293 cell cultures
- Employs the same transient expression protocols typically used in current low-density 293 suspension culture systems to easily switch from low density systems to the high yield, high density Expi293™ Expression System
- Provides robust and reproducible transfection results, giving users greater confidence in their results
- Scale transfections for culture volumes of less than 1 mL to greater than 10 liters, while maintaining equivalent volumetric protein yields
The ExpiFectamine™ 293 Transfection Kit is specifically designed to work with Expi293F™ cells in the Expi293™ Expression System. The transfection kit consists of the cationic lipid-based ExpiFectamine™ 293 Reagent and ExpiFectamine™ 293 Transfection Enhancers 1 and 2. The enhancers are added 18 to 22 hours post-transfection to boost transfection, cell viability, and protein expression. The protocol for use of ExpiFectamine™ 293 Transfection components is very similar to transfection protocols for other transient expression reagents, such as 293fectin™ and FreeStyle™ MAX.
Specifications
Expi293F™ | |
Established Cell Lines | |
Lipid-based Transfection | |
ExpiFectamine™ | |
Cell Culture Grade |
Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free | |
Plasmid DNA | |
Transfection | |
Transfection Kit | |
No |
For Research Use Only. Not for use in diagnostic procedures.