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Invitrogen™ F(ab)-Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody

Produktkod. 31781052
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Kvantitet:
250 μL
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Produktkod. Kvantitet
31781052 250 μL
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Produktkod. 31781052 Leverantör Invitrogen™ Leverantörsnummer SA510397
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Beräknad leverans: 13-10-2026
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Beskrivning

F(ab)-Goat Recombinant Superclonal Secondary Antibody

F(ab)-Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody can detect all subtypes of Mouse IgG (Mouse IgG1,2a,2b). It showed minimum to no cross-reactivity to various species immunoglobulins tested. This Fab fragment antibody is expressed in vitro and purified as a recombinant construct. It is also engineered to deliver enhanced stability and sensitivity. The advantages of recombinant antibodies include-better specificity, animal origin-free formulation, and lot-to-lot consistency. F(ab) fragments offer several advantages over full-length IgGs due to their smaller molecular size (~50 kDa vs. ~150 kDa), such as improved tissue penetration. The absence of the Fc region reduces nonspecific binding and background staining in Fc receptor-expressing cells and tissues, while also enabling increased flexibility in multiplexing assays. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
TRUSTED_SUSTAINABILITY

Specifikationer

Antigen Mouse IgG (H+L)
Användningsområden ELISA, Western Blot, Immunocytochemistry
Klassificering Recombinant Superclonal
Koncentration 1 mg/mL
Konjugera Unconjugated
Formulering PBS with 0.09% sodium azide; pH 7.2-7.4
Värd art Goat
Immunogen Purified Mouse IgG.
Reningsmetod Affinity chromatography
Kvantitet 250 μL
Regulatorisk status RUO
Primär eller sekundär Secondary
Målarter Mouse
Innehåll och lagring Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Produkttyp Secondary Antibody
Form Liquid
Isotyp IgG F(ab)
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