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F4/80, eFluor 570, clone: BM8, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 41-4801-80

Additional Details : Weight : 0.09500kg

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Product Code. 15508946

  • 1046.00 SEK / 25µg


EU Stock 4
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Description

Description

Description: The BM8 monoclonal antibody reacts with mouse F4/80 antigen, an approximately 160kDa surface receptor. It belongs to the EGF-TM7 family of proteins. As such it contains seven EGF-like domains on its extracellular N-terminus, seven transmembrane spanning sequences, and an intracellular C-terminal domain showing homology to other TM7 superfamily members. The F4/80 antigen is expressed by a majority of mature macrophages and is one of the best markers for this population of cells. However, other cell types, such as peritoneal eosinophils, Langerhans cells, and some other dendritic cell subtypes, have been reported to express this antigen as well. Expression of F4/80 commences during early myeloid development in vivo and can be upregulated on BM cells stimulated in vitro with M-CSF. Some populations of macrophages, especially in the lymphoid microenvironment, may be devoid of F4/80.Applications Reported: This BM8 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy.Applications Tested: This BM8 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using pronase antigen retrieval and can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.Excitation: 555 nm; Emission: 570 nm
Specifications

Specifications

F4/80 Antigen
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q61549
DD7A5-7, EGF-TM7, F4/80, Gpf480, Ly71, TM7LN3
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
ImmunohistochemistryþImmunohistochemistry (Frozen)þMicroscopy
BM8
eFluor 570
Adgre1
Conjugated
Adgre1
IgG2a, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
13733
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