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F4/80 Monoclonal Antibody (BM8), Super Bright™ 645, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 64-4801-82
Additional Details : Weight : 0.01000kg
Description
Description: The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin. Applications Reported: This eBio299Arm antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio299Arm antibody has been tested by flow cytometric analysis of mouse splenocytes. Use isotype control at the same concentration as experimental antibody. Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No. SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
F4/80 Monoclonal antibody specifically detects F4/80 in Mouse samples. It is validated for Flow Cytometry.Specifications
| F4/80 | |
| Monoclonal | |
| 0.2 mg/mL | |
| PBS with BSA and 0.09% sodium azide; pH 7.2 | |
| Q61549 | |
| ADGRE1 | |
| Affinity chromatography | |
| RUO | |
| 13733 | |
| 4° C, store in dark, DO NOT FREEZE! | |
| Liquid |
| Flow Cytometry | |
| BM8 | |
| Super Bright 645 | |
| ADGRE1 | |
| ADGRE1; Adhesion G protein-coupled receptor E1; Adhesion G protein-coupled receptor E2; cell surface glycoprotein EMR1; cell surface glycoprotein F4/80; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like 1; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr1; EMR1 hormone receptor; F4/80; Gpf480; Ly71; lymphocyte antigen 71; TM7LN3 | |
| Rat | |
| 100 μg | |
| Primary | |
| Mouse | |
| Antibody | |
| IgG2a κ |