FceR1 alpha, Functional Grade, clone: MAR-1, eBioscience™
Armenian Hamster Monoclonal Antibody
Brand: Affymetrix eBioscience 16-5898-85
Additional Details : Weight : 0.01000kg
DescriptionDescription: The MAR-1 monoclonal antibody reacts with the Fc epsilon Receptor I alpha subunit, an IgE-binding subunit lacking signal-transducing ability. Fc epsilon RI alpha; is expressed on mast and basophil cells and is up-regulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The Fc epsilon RI complex plays an important role in triggering IgE-mediated allergic reactions.Applications Reported: The MAR-1 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of IgE to Fc epsilon Receptor and degranulation of mast cells.Applications Tested: The MAR-1 antibody has been tested by flow cytometric analysis of the MC/9 cell line (a mouse mast cell line). This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|PBS with no preservative; pH 7.2|
|RP11-550P17.3, FCE1A, FcERI|
|Flow Cytometry, Functional Assay, Neutralization|
|Store at 2-8°C.|
For Research Use Only.