FceR1 alpha, clone: AER-37 (CRA1), eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-5899-80
Additional Details : Weight : 0.01000kg
DescriptionDescription: The AER-37 monoclonal antibody reacts with the FcεRIalpha subunit, an IgE-binding subunit lacking signal-transducing ability. FcεRIalpha is expressed on mast and basophil cells and is upregulated by the presence of IgE. FcεRIalpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The FcεRI complex plays an important role in triggering IgE-mediated allergic reactions.Applications Reported: The AER-37 (CRA1) antibody has been reported for use in flow cytometric analysis. It has been reported to not block IgE binding.Applications Tested: The AER-37 (CRA1) antibody has been tested by flow cytometric analysis of peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|RP11-550P17.3, FCE1A, FcERI|
|IgG2b, kappa, kappa|
For Research Use Only.