Thermo Scientific™ Maxima™ First Strand cDNA Synthesis Kit for RT-qPCR
Perform cDNA synthesis in two step real time quantitative RT-PCR (RT-qPCR) applications with the convenient, optimized system of First Strand cDNA Synthesis Kit for RT-qPCR.
Brand: Thermo Scientific™ K1642
Additional Details : Weight : 0.01000kg
DescriptionThe kit uses Maxima Reverse Transcriptase, an advanced enzyme derived by in vitro evolution of M-MuLV RT. The enzyme features high thermostability, robustness and increased cDNA synthesis rate compared to wild type M-MuLV RT. The contents of the kit include the Maxima Enzyme Mix, 5X Reaction Mix and Water, nuclease-free. Maxima Enzyme Mix contains Maxima Reverse Transcriptase and RiboLock RNase Inhibitor. The recombinant RiboLock RNase Inhibitor effectively protects RNA templates from degradation by RNases A, B and C at temperatures up to 55°C. 5X Reaction Mix contains the remaining reaction components: reaction buffer, dNTPs, oligo (dT)18 and random hexamer primers. Water, nuclease-free is provided for reaction set-up and dilution of sample RNA. The absence of endo-, exodeoxyribonucleases, ribonucleases and phosphatases has been confirmed by appropriate quality tests.
- Sensitive and reproducible cDNA synthesis from a wide range of total RNA amounts (1pg to 5μg)
- Increased synthesis rate—complete cDNA synthesis in 15 minutes
- Increased reaction temperature—up to 60°C
- Convenient format—premixed solutions for use in RT-qPCR
Two step RT-qPCR.
|Two step RT-qPCR.|
|Maxima™ First Strand cDNA Synthesis Kit for RT-qPCR|
This product or the use of this product is covered by international patent application WO/2009/125006. The purchase of this product includes a non-transferable license to use this product for the purchaser's internal research. All other commercial uses of this product, including without limitation product use for diagnostic purposes, resale of product in the original or any modified form or product use in providing commercial services require a separate license from Fermentas.